RESEARCH ARTICLE
Effects of L-amino Acids on Human Peripheral Neutrophil Granulocyte Activation
Sándor Sipka*, 1, Tamás Keresztes2, Ildikó Kovács1, Sándor Sipka Jr2, Sándor Baráth1, Gyula Szegedi1, +, Margit Zeher1, Geza Bruckner3
Article Information
Identifiers and Pagination:
Year: 2014Volume: 8
First Page: 1
Last Page: 7
Publisher Id: TONUTRJ-8-1
DOI: 10.2174/1874288201408010001
Article History:
Received Date: 31/10/2013Revision Received Date: 27/02/2014
Acceptance Date: 03/03/2013
Electronic publication date: 16/5/2014
Collection year: 2014
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective:
The objective was to investigate the early (20 minutes) effects of 21 L-amino acids on the activation of human neutrophils and to determine in healthy individuals the effects of a meal on the 1) number and relative luminescence unit (RLU) of peripheral neutrophils, 2) serum glutamate and glucose levels and 3) mTOR signaling network.
Methods:
The RLU of neutrophils stimulated by Ca2+ ionophor (CaI) and phorbol myristate acetate (PMA) following amino acid supplementation (3 x 10-4 M) or after consuming a meal was determined. L-glutamate was measured by HPLC.
Results:
All amino acids resulted in significant inhibitions of neutrophil RLU, except for arginine, which stimulated neutrophils. The ratios of amino acid induced inhibition were significantly higher in the cells stimulated by PMA than by CaI. The consumption of a meal resulted in a significant serum glutamate elevation compared to baseline (2.3 versus 0.9 x10-4 M) 90 minutes after ingestion of the meal. It was independent of the body mass index and returned near fasting levels after 150 minutes. The number of neutrophils was significantly elevated 90 minutes after the meal but the PMA induced RLU was significantly decreased.
Conclusion:
Our ex vivoand in vivoresults suggest that the L-amino acids, independent of their metabolic significance, may continuosly and quickly modify the activity of human peripheral neutrophils, and also the outcome of various immunologic reactions. The activation of the mTORC1 complex likely involves a transient impairment in the function of mTORC2 complex in these processes.
