RESEARCH ARTICLE


Faecal Contaminants in Edible Bivalves from Maputo Bay, Mozambique: Seasonal Distribution, Pathogenesis and Antibiotic Resistance



Betty Collin1, 2, Ann-Sofi Rehnstam-Holm1, 2, Bodil Hernroth*, 3
1 Department of Biomedicine, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg
2 Department of Mathematics and Natural Sciences, Kristianstad University, 291 88 Kristianstad
3 The Royal Swedish Academy of Sciences, Kristineberg 566, 450 34 Fiskebäckskil, Sweden


© 2008 Collin et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Royal Swedish Academy of Sciences, Kristineberg 566, 450 34 Fiskebäckskil, Sweden; Tel: +46 523 18513; Fax: +46 523 18502; E-mail:bodil.hernroth@marecol.gu.se


Abstract

In Maputo, Mozambique marine bivalves considerably contribute to the diet of the population. This study aimed to investigate seasonal distribution of Escherichia coli and Salmonella in clams from Maputo Bay, and examine their pathogenesis and antibiotic-resistant patterns. Standard multiple tube method revealed that the concentration of coliforms in all samples exceeded the limit for direct consumption, according to EU standards. Thirty-eight percent of the samples contained >60,000 MPN per 100 gram flesh. The occurrence of E. coli did not differ significantly due to season, while Salmonella was present in 100% of the samples during the rainy period and only in 30% during the dry. Multiplex polymerase chain reaction showed that 45% of E. coli isolates were positive for the virulent indicator gene fimA. The Salmonella isolates were identified as S. enterica serovar Typhimurium. Among other isolated coliformic Enterobacteriaceae, Shigella sp. (specie), which in low doses can cause severe gastrointestinal infections, was identified. Antimicrobial susceptibility, recorded by the disk diffusion method, showed resistance to the most commonly used antibiotics. This high levels of faecal contaminants in the clams points out the need for risk assessment and sanitary improvements.